this weekend i was treated to an escape from toronto courtesy of my landlords. they had been encouraging me to join them at their house in collingwood for the past few weeks, and the timing appeared to be right for this particular saturday/sunday. the only condition was full internet access, as i have been going back and forth with my supervisor on final details for his grant proposal that is due next wednesday. fortunately, the internet was not a problem, and i have fully enjoyed my relaxing winter saturday away from the city. i started by waking up naturally with the sun at 8:00 am (something not often possible in my basement apartment), then enjoyed a freshly baked muffin and coffee for breakfast. took a leisurely shower after reading the entire globe & mail. worked on aforementioned grant for a couple of hours and paused for lunch. then, i snowshoed up the escarpment and around a loop of the bruce trail with my landlord, which took about 2 hours. great cardio (!) and so peaceful in the ravines. i thought about taking my camera, but didn't want to spoil the serenity of the run by stopping to take snaps. so unfortunately, no photos for all of you. the view from the top was incredible though - at one point you can see georgian bay out between two of the ski hills. after the very quick trip down the escarpment, i took a 30 minute soak in the outdoor hot tub. outdoor hot tubs are SO underrated. after another shower and another hour of work i helped assemble a few parts of dinner, and spent the evening with friends of the landlords. i think tomorrow i will try for another snowshoe run up the hills, justifying another hot tub soak. then i should get back to the city and back to reality. not for long though, as i'm headed for vancouver on thursday morning!
that's all. snowshoes plus hot tub soak plus wine equals sleepy.
24.2.07
13.2.07
sub-zero neuron tracing
it is cold in toronto. i'm not complaining, but merely informing you that it hurts to be outside right now. of course, this snap has timed itself perfectly for the one month in which i need to spend more time than usual walking around out of doors. i'm in the last stretch of an experiment that i started back in november, and this particular phase involves using a microscope located at the Toronto Western Hospital. my lab is at a different hospital, about a 12 minute walk from where the microscope is. unfortunately, those 12 minutes are not in the direction of my house, but further away from my house. this means that every morning i have to spend 32 minutes walking to work instead of 20, and then there are one or two 24 minute return trips to the lab squished in over the day. i have broken out the long johns.
incidentally, i still wear the same long johns that i've had since elementary school. somehow they wield a miraculous power of stretch, while maintaining consistent warm-and-cosiness.
anyways, for the past couple of weeks i have been tracing neurons. apart from eating, this is how i kept myself busy while in India last winter (as described here). in fact, the reason that i was sent to India in the first place was to learn a method of staining tissue called Golgi-Cox impregnation. this Golgi Staining allows you to visualize single neurons in a thick section of brain tissue. then, with fancy computer software and a microscope, you can trace the outline of the neuron's body (called the soma in this context) and all of its arms and legs (called dendrites). you end up with something that looks like this:
then, with the fancy computer software, i can determine how long the arms and legs are and how big the cell body is. i might also get excited and look at how complicated the network of arms and legs is. some neurons will have only one or two arms, while others will have many. this tells us a bit about how active the cell might have been while it was still alive - a neuron with fewer arms would make fewer points of contact with other neurons, while a neuron with plenty of arms and legs would probably have been heavily connected to other neurons.
because they're so pretty, here's one more before i brave the cold:
incidentally, i still wear the same long johns that i've had since elementary school. somehow they wield a miraculous power of stretch, while maintaining consistent warm-and-cosiness.
anyways, for the past couple of weeks i have been tracing neurons. apart from eating, this is how i kept myself busy while in India last winter (as described here). in fact, the reason that i was sent to India in the first place was to learn a method of staining tissue called Golgi-Cox impregnation. this Golgi Staining allows you to visualize single neurons in a thick section of brain tissue. then, with fancy computer software and a microscope, you can trace the outline of the neuron's body (called the soma in this context) and all of its arms and legs (called dendrites). you end up with something that looks like this:
then, with the fancy computer software, i can determine how long the arms and legs are and how big the cell body is. i might also get excited and look at how complicated the network of arms and legs is. some neurons will have only one or two arms, while others will have many. this tells us a bit about how active the cell might have been while it was still alive - a neuron with fewer arms would make fewer points of contact with other neurons, while a neuron with plenty of arms and legs would probably have been heavily connected to other neurons.
because they're so pretty, here's one more before i brave the cold:
5.2.07
i heart co-op
apparently the excitement of my new laptop has kept me from posting for a full 2.5 weeks. i've been amassing things to talk about in that time, though, so sit tight.
first up? my new obsession and life-changing experience. joining a neighbourhood food co-op! my landlords were generously kind enough to gift me with a membership for xmas. i had been talking to one of them about the struggle to find good local produce on a regular and accessible basis without signing up for a delivery service or visiting the farmer's market on saturday mornings. she had been a member of a local co-op for the past many years, and suggested i come try it out. in my 2006 procrastinating ways, i never did. but with the gifting of the membership, i was obligated - and so, about a week after i got back to toronto from the holidays, i made my way over to the co-op and executed my first trial shop. i was hardly in the doors before falling head over heels. not only is it hidden in a little back alley, away from the noise and light pollution of the big city, but real people work there (i.e. not teenage robots) and they play the same music that i was listening to at home 10 minutes before walking over!
before extolling any more virtues, i will explain to my questioning readers what exactly 'food co-op' refers to. in this case, a group of people got together back in the 70's and felt like they were not having sufficient access to healthy, locally-grown, non-pesticided foods. these people took a mortgage out on an old bakery, started sourcing healthy, locally-grown, non-pesticided foods, and opened up to the community. in order to shop at the co-op, you had to become a member. this meant paying a small membership loan up front (it's now about $70), an annual membership fee (now about $16), and volunteering 2 hours of your time every month to help keep the place running. in return, the co-op staff take care of making sure the food is grown, prepared, and packaged in a healthy and ethical way, and they keep mark-up on prices incredibly low because the co-op is NON-PROFIT. so much like for one of my favourite NON-PROFIT orgs from one of my earlier lives, the Ottawa School of Art (where I worked for several years during high school), all money that the co-op takes in is invested back in the co-op. as a result, money flows from the customer through the co-op to the healthy and ethical food producers, and the producers get to remain producers as they are sustained by support from the co-op.
all this appeals to me at a political level and at a basic satisfaction of needs level. as someone who enjoys food A LOT, and who loves to cook in a number of different styles, i am blown away by the diversity of ingredients the co-op carries. at last! one store that sells Lacinato kale, shiitake mushrooms, tamari-roasted almonds, and my favourite breakfast muslix pitas. behold the bounty.
and that was just my trial shop...
first up? my new obsession and life-changing experience. joining a neighbourhood food co-op! my landlords were generously kind enough to gift me with a membership for xmas. i had been talking to one of them about the struggle to find good local produce on a regular and accessible basis without signing up for a delivery service or visiting the farmer's market on saturday mornings. she had been a member of a local co-op for the past many years, and suggested i come try it out. in my 2006 procrastinating ways, i never did. but with the gifting of the membership, i was obligated - and so, about a week after i got back to toronto from the holidays, i made my way over to the co-op and executed my first trial shop. i was hardly in the doors before falling head over heels. not only is it hidden in a little back alley, away from the noise and light pollution of the big city, but real people work there (i.e. not teenage robots) and they play the same music that i was listening to at home 10 minutes before walking over!
before extolling any more virtues, i will explain to my questioning readers what exactly 'food co-op' refers to. in this case, a group of people got together back in the 70's and felt like they were not having sufficient access to healthy, locally-grown, non-pesticided foods. these people took a mortgage out on an old bakery, started sourcing healthy, locally-grown, non-pesticided foods, and opened up to the community. in order to shop at the co-op, you had to become a member. this meant paying a small membership loan up front (it's now about $70), an annual membership fee (now about $16), and volunteering 2 hours of your time every month to help keep the place running. in return, the co-op staff take care of making sure the food is grown, prepared, and packaged in a healthy and ethical way, and they keep mark-up on prices incredibly low because the co-op is NON-PROFIT. so much like for one of my favourite NON-PROFIT orgs from one of my earlier lives, the Ottawa School of Art (where I worked for several years during high school), all money that the co-op takes in is invested back in the co-op. as a result, money flows from the customer through the co-op to the healthy and ethical food producers, and the producers get to remain producers as they are sustained by support from the co-op.
all this appeals to me at a political level and at a basic satisfaction of needs level. as someone who enjoys food A LOT, and who loves to cook in a number of different styles, i am blown away by the diversity of ingredients the co-op carries. at last! one store that sells Lacinato kale, shiitake mushrooms, tamari-roasted almonds, and my favourite breakfast muslix pitas. behold the bounty.
and that was just my trial shop...
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